# Biopython – Pairwise Alignment

• Last Updated : 28 Jul, 2022

Pairwise Sequence Alignment is a process in which two sequences are compared at a time and the best possible sequence alignment is provided. Pairwise sequence alignment uses a dynamic programming algorithm. Biopython has a special module Bio.pairwise2 which identifies the alignment sequence using pairwise method. Biopython provides the best algorithm to find alignment sequence as compared to other software.

Let’s take two simple and hypothetical sequences as an example for using pairwise module.

## Python3

 `# Import libraries``from` `Bio ``import` `pairwise2``from` `Bio.Seq ``import` `Seq` `# Creating sample sequences``seq1 ``=` `Seq(``"TGTGACTA"``)``seq2 ``=` `Seq(``"CATGGTCA"``)` `# Finding similarities``alignments ``=` `pairwise2.align.globalxx(seq1, seq2)` `# Showing results``for` `match ``in` `alignments:``    ``print``(match)`

Output:

Here, the globalxx method does the main work, it follows the convention <alignment type>XX where XX is a code having two characters indicating the parameters it takes. First character indicates the matching and mismatching score while the second indicates the parameter for the gap penalty.

Match parameters :

gap penalty parameters :

For a nice printout Bio.pairwise2 provides format_alignment() method:

## Python3

 `# Import libraries``from` `Bio ``import` `pairwise2``from` `Bio.Seq ``import` `Seq``from` `Bio.pairwise2 ``import` `format_alignment` `# Creating sample sequences``seq1 ``=` `Seq(``"TGTGACTA"``)``seq2 ``=` `Seq(``"CATGGTCA"``)` `# Finding similarities``alignments ``=` `pairwise2.align.globalxx(seq1, seq2)` `# Showing results``for` `alignment ``in` `alignments:``    ``print``(format_alignment(``*``alignment))`

Output:

There is another module provided by Biopython to do the pairwise sequence alignment. Align module has a PairwiseAligner() for this purpose. It has various APIs to set the parameters like mode, match score, algorithm, gap penalty, etc. Below is a simple implementation of the method:

## Python3

 `# Import libraries``from` `Bio ``import` `Align``from` `Bio.Seq ``import` `Seq` `# Creating sample sequences``seq1 ``=` `Seq(``"TGTGACTA"``)``seq2 ``=` `Seq(``"CATGGTCA"``)` `# Calling method``aligner ``=` `Align.PairwiseAligner()` `# Showing method attributes``print``(aligner)` `# Finding similarities``alignments ``=` `aligner.align(seq1, seq2)` `# Showing results``for` `alignment ``in` `alignments:``    ``print``(alignment)`

Output:

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